This is a description of how FamLink can be used to calculate the LR for a case involving two linked markers and the hypotheses H1: grandparent - grandchild versus H2:Unrelated. Genetic data for a child and the alleged grandchild exist. (Below you are guided to provide input in a way which allows for the result to be checked against a theoretical result in the paper by Kling, Egeland and Tillmar, 2012). 1. Open FamLink. 2. Click on the Frequency database option in the File menu. 3. Click the Option tab. The Error rate should be set to 0 and Theta(r) to 0.1. 4. Save and close the Frequency database option and click on New wizard in the File menu. 5. Select the Grandparent pedigree as the basic hypothesis and click next. (There are several similar pedigrees and you should choose the one with the precise text Grandparent under). 6. Choose Unrelated man in the next dialog as the alternative hypothesis. Move on by clicking next. 7. First select 1.NN, which corresponds to the alleged grandparent, and enter the DNA-data by using the drop down menu. Select the first allele in the drop down menu in all cases. Next select 2.NN and assign alleles which differ from those of the first individual for instance by selecting the second allele in the drop down menu in all cases. 8. Click next and click on the Calculate tab. The likelihood for the hypotheses, and the corresponding LR, is calculated. When you click on Grandparent pedigree you should see the output LR(Linkage): 0.45 LR(No linkage): 0.25 9. The result can be stored for instance by choosing Generate report- A more comprehensive description of all the features of FamLink, and more examples, can be found in the manual.
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